| 1 | Joyce Wolters (A1) | NED | 43.46 | PB | - |
| 2 | Sandra Dekker (C2) | NED | 43.50 | SB | +0.040 |
| 3 | Gerdien Meijer-Verweij (M45) | NED | 43.57 | | +0.110 |
| 4 | Anouk Tjepkema (B1) | NED | 43.95 | | +0.490 |
| 5 | Eva van den Born (Neo) | NED | 43.96 | | +0.500 |
| 6 | Renée Barendregt (B1) | NED | 44.22 | PB | +0.760 |
| 7 | Demi Meek (C2) | NED | 44.24 | SB | +0.780 |
| 8 | Suzanne Mulder (M30) | NED | 44.69 | SB | +1.230 |
| 9 | Edel Jasmijn Bording (B2) | NED | 44.78 | | +1.320 |
| 10 | Daniëlle Hoogendoorn (B2) | NED | 44.88 | | +1.420 |
| 11 | Anna Wester (B1) | NED | 44.96 | | +1.500 |
| 12 | Robin van Leeuwen (B2) | NED | 45.13 | | +1.670 |
| 13 | Annemarie Roozen (B2) | NED | 45.38 | | +1.920 |
| 14 | Famke Wilmink (C2) | NED | 45.63 | | +2.170 |
| 15 | Quinty Ton (C2) | NED | 46.03 | SB | +2.570 |
| 16 | Sandra Bijlert-Burgerhout (M35) | NED | 46.10 | | +2.640 |
| 17 | Dominique de Beer (C2) | NED | 46.45 | | +2.990 |
| 18 | Lian van Dongen (M50) | NED | 47.12 | | +3.660 |
| 19 | Emma Kostelijk (C2) | NED | 47.19 | PB | +3.730 |
| 20 | Kika Banning (B1) | NED | 48.14 | | +4.680 |
| DNF | Charlotte Kool (C2) | NED | DNF | | - |