| 1 | Anne Ruyssenaars (A1) | NED | 42.80 | | - |
| 2 | Marjolein van der Steen (Neo) | NED | 42.84 | | +0.043 |
| 3 | Naomi van der Werf (B2) | NED | 43.92 | | +1.128 |
| 4 | Roxane Demmink (B1) | NED | 44.14 | | +1.340 |
| 5 | Myrthe Drogtrop (A2) | NED | 44.89 | | +2.094 |
| 6 | Janneke Hennipman (A1) | NED | 46.16 | | +3.369 |
| 7 | Jolien van den Berg (Senior) | NED | 46.27 | SB | +3.472 |
| 8 | Sara van Vliet (B2) | NED | 46.88 | | +4.082 |
| 9 | Allessandra Primavera (C2) | NED | 47.50 | SB | +4.709 |
| 10 | Ine Bonthuis (C2) | NED | 47.56 | | +4.762 |
| 11 | Vera van Ditshuizen (D2) | NED | 47.72 | | +4.922 |
| 12 | Florien Bolks (C2) | NED | 49.58 | SB | +6.780 |
| 13 | Minou Groenen (Neo) | NED | 51.63 | | +8.838 |
| 14 | Monique van Dorresteijn (B1) | NED | 53.08 | SB | +10.284 |