| 1 | Isabelle Hersbach (Senior) | NED | 44.21 | | - |
| 2 | Heleen Docter (Senior) | NED | 46.74 | SB | +2.530 |
| 3 | Mariska van Zon (Senior) | NED | 47.27 | | +3.060 |
| 4 | Noa Halma (D2) | NED | 47.66 | | +3.450 |
| 5 | Tessa.13 Bos (D2) | NED | 49.41 | | +5.200 |
| 6 | Marcella van de Weem (Neo) | NED | 49.95 | SB | +5.740 |
| 7 | Roos Toonen (E2) | NED | 50.13 | | +5.920 |
| 8 | Lisa Rimmelzwaan (D1) | NED | 50.33 | SB | +6.120 |
| 9 | Fenna Stougje (D2) | NED | 51.79 | PB | +7.580 |
| 10 | Ellen Govaert (D2) | NED | 51.95 | SB | +7.740 |
| 11 | Tess van der Salm (D2) | NED | 53.83 | SB | +9.620 |
| 12 | Sara Van Schie (D1) | NED | 53.91 | PB | +9.700 |
| 13 | Guusje Spaargaren (D2) | NED | 54.54 | PB | +10.330 |
| 14 | Annewil Stelloo (M40) | NED | 54.66 | SB | +10.450 |
| 15 | Amelia McCormack (Senior) | GBR | 54.70 | PB | +10.490 |
| 16 | Anna Hogendoorn (E2) | NED | 55.50 | PB | +11.290 |
| 17 | Tessa Verboom (D1) | NED | 55.70 | | +11.490 |
| 18 | Fien Moerman (E2) | NED | 56.05 | PB | +11.840 |
| 19 | Jo-Lien van Scheijndel (E2) | NED | 1:01.91 | SB | +17.700 |
| 20 | Jane van Nierop (D1) | NED | 1:02.90 | SB | +18.690 |
| 21 | Celine van Laar (E2) | NED | 1:03.58 | SB | +19.370 |
| 22 | Jaelyn Silva (E2) | NED | 1:11.14 | SB | +26.930 |
| DNS | Mijke Anne Kanneworff (M40) | NED | DNS | | - |