| 1 | Wataru Morishige (Senior) | JPN | 34.57 | SB | - |
| 2 | Katsuhiro Kuratsubo (Senior) | JPN | 34.82 | SB | +0.250 |
| 3 | Yuuma Murakami (Senior) | JPN | 34.83 | | +0.260 |
| 4 | Yuuta Hirose (Neo) | JPN | 34.95 | SB | +0.380 |
| 5 | Tatsuya Shinhama (Senior) | JPN | 34.98 | SB | +0.410 |
| 6 | Ryota Kojima (Senior) | JPN | 35.01 | SB | +0.440 |
| 7 | Shougo Takamizawa (Neo) | JPN | 35.13 | PB | +0.560 |
| 8 | Taiyo Nonomura (Senior) | JPN | 35.15 | SB | +0.580 |
| 9 | Issa Gunji (Neo) | JPN | 35.22 | | +0.650 |
| 10 | Yuudai.02 Yamamoto (Senior) | JPN | 35.31 | SB | +0.740 |
| 11 | Shouta Tsubaki (Senior) | JPN | 35.35 | SB | +0.786 |
| 12 | Yamato Matsui (Senior) | JPN | 35.35 | SB | +0.787 |
| 13 | Fuga Tsujimoto (Neo) | JPN | 35.43 | SB | +0.860 |
| 14 | Souki Ooshima (Senior) | JPN | 35.45 | SB | +0.880 |
| 15 | Takuma Kawame (Senior) | JPN | 35.52 | SB | +0.950 |
| 16 | Takuya Morimoto (Senior) | JPN | 35.56 | SB | +0.990 |
| 17 | Motoki Abe (Neo) | JPN | 35.57 | SB | +1.000 |
| 18 | Itsushi Tsujimoto (Senior) | JPN | 35.83 | | +1.260 |