| 1 | Jade Holborough-Kerkvliet (C1) | NED | 47.61 | | - |
| 2 | Lianne Koetsier (C1) | NED | 47.70 | SB | +0.086 |
| 3 | Mirthe Paul (C2) | NED | 48.75 | | +1.135 |
| 4 | Irem Kaya (C1) | NED | 49.23 | SB | +1.615 |
| 5 | Annemarie Kersbergen (C2) | NED | 51.35 | | +3.736 |
| 6 | Belinda Kleijn (C2) | NED | 52.65 | | +5.037 |
| 7 | Anique van der Greft (A1) | NED | 53.71 | SB | +6.097 |
| 8 | Chiara de Lange (B1) | NED | 53.93 | PB | +6.317 |
| 9 | Hannah Treep (A1) | NED | 54.26 | | +6.646 |
| 10 | Eline Koetsier (B1) | NED | 55.42 | SB | +7.804 |
| 11 | Maartje Zaalberg (C2) | NED | 56.01 | PB | +8.395 |
| 12 | Denise Slappendel (C1) | NED | 57.49 | SB | +9.879 |
| 13 | Aniek Heemskerk (C2) | NED | 59.77 | PB | +12.159 |
| 14 | Maaike Doornheim (C1) | NED | 59.96 | SB | +12.343 |
| 15 | Inge.01 Kuiper (C2) | NED | 1:00.34 | SB | +12.723 |
| 16 | Roos Ruitenbeek (D1) | NED | 1:00.98 | SB | +13.367 |
| 17 | Cathrien de Blois (M45) | NED | 1:01.76 | SB | +14.148 |
| 18 | Megan Berhouwer (B1) | NED | 1:03.91 | | +16.293 |
| 19 | Marlous Elstgeest (M35) | NED | 1:04.32 | PB | +16.705 |
| 20 | Liselotte Boinck (D2) | NED | 1:04.68 | | +17.063 |
| 21 | Sabine van der Voort (B1) | NED | 1:06.39 | SB | +18.772 |
| 22 | Beate Bergman (B1) | NED | 1:13.62 | SB | +26.011 |
| 23 | Emma Ruitenbeek (C2) | NED | 1:15.60 | SB | +27.983 |
| 24 | Caroline de Kleer (M55) | NED | 1:18.07 | PB | +30.459 |
| 25 | Anneke Brouwer (M55) | NED | 1:18.73 | SB | +31.120 |
| DNS | Inge de Heer (C2) | NED | DNS | | - |