| 1 | Famke Minnee (B2) | NED | 42.19 | | - |
| 2 | Antoinet van Essen-Prins (Senior) | NED | 43.49 | PB | +1.300 |
| 3 | Lara van Son (C2) | NED | 44.09 | | +1.900 |
| 4 | Stephanie Bunte (Senior) | NED | 44.47 | SB | +2.280 |
| 5 | Anke Breunis (Neo) | NED | 44.63 | | +2.440 |
| 6 | Fabienne Diniz (C2) | NED | 44.65 | | +2.460 |
| 7 | Birte Tellegen (C1) | NED | 45.11 | | +2.920 |
| 8 | Dorianne Kok (Senior) | NED | 45.37 | PB | +3.180 |
| 9 | Marte Kloosterman (B2) | NED | 46.04 | PB | +3.850 |
| 10 | Ellemijn Luisman (C2) | NED | 46.22 | | +4.030 |
| 11 | Robin Keppels (D2) | NED | 46.32 | SB | +4.130 |
| 12 | Bo Meijer (C2) | NED | 46.68 | SB | +4.490 |
| 13 | Britt Bijker (C1) | NED | 46.70 | SB | +4.510 |
| 14 | Emma van der Lied (C2) | NED | 46.77 | | +4.580 |
| 15 | Sophie Exterkate (D1) | NED | 46.97 | | +4.780 |
| 16 | Jasmijn Polman (C2) | NED | 47.11 | | +4.920 |
| 17 | Britt van der Linden (D1) | NED | 47.68 | | +5.490 |
| 18 | Anne-Fleur van der Lied (D1) | NED | 48.76 | SB | +6.570 |
| 19 | Marianna ter Schure (Senior) | NED | 49.79 | SB | +7.600 |
| 20 | Anouk Kraa (Neo) | NED | 50.24 | PB | +8.050 |
| 21 | Kris Wieringa (B2) | NED | 50.27 | PB | +8.080 |
| 22 | Maureen Vos (B2) | NED | 50.97 | SB | +8.780 |
| 23 | Astrid Minnee (M45) | NED | 51.25 | PB | +9.060 |
| 24 | Marit de Ruiter (C1) | NED | 51.88 | | +9.690 |
| 25 | Ilse Hofstee (B2) | NED | 51.98 | SB | +9.790 |
| 26 | Mendy Pasman (C2) | NED | 52.38 | | +10.190 |
| 27 | Kyara Pasman (D1) | NED | 52.46 | SB | +10.270 |
| 28 | Nikki Winter (A2) | NED | 52.58 | | +10.390 |
| 29 | Yonne Oonk (D1) | NED | 52.60 | PB | +10.410 |
| 30 | Anne Keppels (E1) | NED | 53.66 | | +11.470 |
| 31 | Noor Wieringa.02 (B1) | NED | 53.93 | PB | +11.740 |
| 32 | Denice Burgmeijer (D2) | NED | 54.55 | SB | +12.360 |
| 33 | Caerys Fransen (D1) | GBR | 54.70 | | +12.510 |
| 34 | Noa Handelzalts (E2) | NED | 55.28 | | +13.090 |
| 35 | Lindsey Andeweg (Senior) | NED | 56.17 | PB | +13.980 |
| 36 | Mette ten Cate (E2) | NED | 57.91 | | +15.720 |
| 37 | Dilara Hultink (E1) | NED | 58.24 | SB | +16.050 |
| 38 | Eva Weultjes (E2) | NED | 1:00.46 | | +18.270 |
| 39 | Anna Super (C1) | NED | 1:01.46 | | +19.270 |
| 40 | Alette Koole (M30) | NED | 1:02.54 | PB | +20.350 |
| 41 | Maud Van Geelen (D1) | NED | 1:05.31 | PB | +23.120 |
| 42 | Suze ten Hartog (E1) | NED | 1:05.52 | SB | +23.330 |
| 43 | Bente Van Bolhuis (E1) | NED | 1:06.74 | | +24.550 |
| 44 | Sarah Kamp (E2) | NED | 1:07.84 | | +25.650 |
| 45 | Marit Bouwman (C1) | NED | 1:08.64 | | +26.450 |
| 46 | Elena Ruijtenberg (E1) | NED | 1:09.18 | SB | +26.990 |
| 47 | Sanna van der Velde (F2) | NED | 1:14.41 | SB | +32.220 |
| 48 | Nova Tieke (E1) | NED | 1:21.60 | SB | +39.410 |
| 49 | Britt Boerrigter (E1) | NED | 1:26.76 | PB | +44.570 |
| DNS | Minou Schoot (C1) | NED | DNS | | - |