| 1 | Victoria Stirnemann (Neo) | GER | 40.64 | SB | - |
| 2 | Laura Kivioja (Neo) | FIN | 40.75 | SB | +0.110 |
| Magdalena Mühle (Neo) | GER | 40.75 | SB | +0.110 |
| 4 | Laethisia Schimek (Senior) | GER | 41.04 | SB | +0.400 |
| 5 | Paula Albrecht (B1) | GER | 41.10 | | +0.460 |
| 6 | Sofiia Khochyna (Neo) | UKR | 41.26 | SB | +0.620 |
| 7 | Lina Poddubnaia (A1) | GER | 41.85 | PB | +1.210 |
| 8 | Luna-Luisa Powik (B2) | GER | 42.03 | SB | +1.390 |
| 9 | Leia Marie Behlau (Senior) | GER | 42.26 | SB | +1.620 |
| 10 | Paula Pfeifer (B2) | GER | 42.33 | | +1.690 |
| 11 | Laura Jankovska (B1) | LAT | 42.36 | SB | +1.720 |
| 12 | Ance Kucina (A1) | LAT | 42.52 | | +1.880 |
| 13 | Lea Martin (A1) | GER | 42.58 | | +1.940 |
| 14 | Mia Meinig (B1) | GER | 42.61 | SB | +1.970 |
| 15 | Tina Hemeltjen (A1) | GER | 42.63 | SB | +1.990 |
| 16 | Frida Brusch (B1) | GER | 42.87 | | +2.230 |
| 17 | Franziska Riedel (B2) | GER | 42.89 | | +2.250 |
| 18 | Neele Göpelt (C2) | GER | 43.08 | SB | +2.440 |
| 19 | Alexandra Sauer (B1) | GER | 43.34 | SB | +2.700 |
| 20 | Maike Pruss (A2) | GER | 43.44 | SB | +2.800 |
| 21 | Sofia Saronni (Neo) | ITA | 43.53 | | +2.890 |
| 22 | Isabelle Duft (B2) | GER | 43.60 | SB | +2.960 |
| 23 | Alicia Sommer (A2) | GER | 43.64 | SB | +3.000 |
| 24 | Anik Podufall (B1) | GER | 43.89 | | +3.250 |
| 25 | Anna Diatchenko (C2) | GER | 44.00 | SB | +3.360 |
| 26 | Mara Fuhrmann (B2) | GER | 44.01 | SB | +3.370 |
| 27 | Nele Charline Neumann (A1) | GER | 44.56 | | +3.920 |
| 28 | Johanna Trapp (B1) | GER | 44.65 | SB | +4.010 |
| 29 | Mika Louise Brandt (C2) | GER | 44.66 | SB | +4.020 |
| 30 | Sahra Michelle Henkel (B1) | GER | 44.95 | | +4.310 |
| 31 | Luisa Jarmatz (B1) | GER | 45.00 | SB | +4.360 |
| 32 | Emma Schöler (C2) | GER | 45.10 | SB | +4.460 |
| 33 | Lena Mothes (C1) | GER | 45.16 | SB | +4.520 |
| 34 | Carla Albrecht (C1) | GER | 45.17 | SB | +4.530 |
| 35 | Alexa Dehs (C1) | GER | 45.21 | PB | +4.570 |
| 36 | Julia Geutner (A1) | GER | 45.29 | | +4.650 |
| 37 | Alice Marletti (A2) | ITA | 45.63 | SB | +4.990 |
| 38 | Marta Otto (C1) | GER | 45.85 | SB | +5.210 |
| 39 | Irene Hörnschemeyer (B1) | GER | 45.95 | | +5.310 |
| 40 | Annalina Müller (B1) | GER | 46.08 | SB | +5.440 |
| 41 | Josephine Helbig (C1) | GER | 46.15 | SB | +5.510 |
| 42 | Henriette Heß (C1) | GER | 46.16 | SB | +5.520 |
| 43 | Claire Tuchscherer (C2) | GER | 46.21 | SB | +5.570 |
| 44 | Florentine Blümel (C2) | GER | 46.22 | | +5.580 |
| 45 | Lilli Malin Mittag (C1) | GER | 46.24 | | +5.600 |
| 46 | Johanna Brückner (C1) | GER | 46.26 | SB | +5.620 |
| 47 | Hannah Krompaß (C1) | GER | 46.27 | PB | +5.630 |
| 48 | Leni Jahnke (C2) | GER | 46.31 | SB | +5.670 |
| 49 | Alina Kebsch (C2) | GER | 46.50 | PB | +5.860 |
| 50 | Kassandra Marzyk (C1) | GER | 46.72 | SB | +6.080 |
| 51 | Luise Stauch (B1) | GER | 47.02 | | +6.380 |
| 52 | Gloria Herold (C2) | GER | 47.14 | | +6.500 |
| 53 | Anna Krömer (A1) | GER | 47.20 | SB | +6.560 |
| 54 | Lilly Engelhardt (C1) | GER | 47.70 | | +7.060 |
| 55 | Lissy Junge (C1) | GER | 48.05 | SB | +7.410 |
| 56 | Magdalena Burau (C1) | GER | 48.32 | SB | +7.680 |
| 57 | Ella Schkutek (C1) | GER | 48.48 | | +7.840 |
| 58 | Fiona Leewen (C2) | GER | 48.83 | SB | +8.190 |
| 59 | Isa de Souza Ferraz Höllein (C2) | GER | 48.93 | | +8.290 |
| 60 | Emely Schürmann (B1) | GER | 49.29 | | +8.650 |
| 61 | July Seifert (C1) | GER | 49.54 | | +8.900 |
| 62 | Antonina Schmidt (C1) | GER | 51.52 | | +10.880 |
| 63 | Elisa Spiegel (B1) | GER | 51.95 | PB | +11.310 |
| DNS | Sarah Rosner (B1) | AUT | DNS | | - |