| 1 | Natascha De Boer (Senior) | NED | 52.90 | PB | - |
| 2 | Marjet Janssen (Senior) | NED | 55.65 | SB | +2.750 |
| 3 | Fimke Anna Giliam (Neo) | NED | 55.70 | | +2.800 |
| 4 | Lotte Valk (Neo) | NED | 57.48 | SB | +4.580 |
| 5 | Hedwig Fossen (Neo) | NED | 57.84 | PB | +4.940 |
| 6 | Lisanne van der Werf (Neo) | NED | 58.86 | SB | +5.960 |
| 7 | Berber Wierda (n/a) | NED | 1:00.00 | PB | +7.100 |
| 8 | Rian Lenting (Senior) | NED | 1:00.78 | PB | +7.880 |
| 9 | Adrianne de Roo (Neo) | NED | 1:00.85 | | +7.950 |
| 10 | Nienke Markensteijn (Neo) | NED | 1:00.97 | | +8.070 |
| 11 | Eva Mulder (Neo) | NED | 1:02.32 | SB | +9.420 |
| 12 | Marlies Meier (Senior) | NED | 1:04.81 | PB | +11.910 |
| 13 | Gwen Oudshoorn (D2) | NED | 1:09.11 | SB | +16.210 |
| 14 | Lisa Mulder (Neo) | NED | 1:10.83 | SB | +17.930 |
| 15 | Wendy Van Roosmalen (Neo) | NED | 1:11.56 | SB | +18.660 |
| 16 | Dagmar Mul (Neo) | NED | 1:12.18 | SB | +19.280 |