| 1 | Jacqueline van Straaten (M50) | NED | 52.86 | SB | - |
| 2 | Joanne Lampen (M35) | NED | 53.63 | SB | +0.770 |
| 3 | Martine Hogendoorn (Senior) | NED | 53.95 | PB | +1.090 |
| 4 | Clarine Bronkhorst (M45) | NED | 56.84 | SB | +3.980 |
| 5 | Gerlinde Paas (M45) | NED | 58.67 | SB | +5.810 |
| 6 | Annemieke de Klein (Senior) | NED | 1:02.48 | SB | +9.620 |
| 7 | Desiree Bonouvrie (M55) | NED | 1:04.72 | SB | +11.860 |
| 8 | Tanja van Oers-Verschuren (M40) | NED | 1:05.62 | SB | +12.760 |
| 9 | Wyke Jouwsma (M50) | NED | 1:06.29 | SB | +13.430 |
| 10 | Gerda Vergeer (M65) | NED | 1:06.71 | SB | +13.850 |
| 11 | Diddy Harskamp-Veenendaal (Senior) | NED | 1:07.10 | SB | +14.240 |
| 12 | N. N. (Senior) | NED | 1:07.45 | PB | +14.590 |