| 1 | Marjolein van der Steen (Neo) | NED | 42.88 | | - |
| 2 | Benita de Man (Senior) | NED | 43.87 | SB | +0.990 |
| 3 | Myrthe Drogtrop (Neo) | NED | 44.61 | SB | +1.730 |
| 4 | Marijke Rempe (A1) | NED | 45.98 | SB | +3.100 |
| 5 | Allessandra Primavera (B1) | NED | 46.41 | | +3.530 |
| 6 | Sophie Rijnders (A1) | NED | 47.32 | | +4.440 |
| 7 | Sarah Vonk (B1) | NED | 47.93 | PB | +5.050 |
| 8 | Annemee Gerritsen (A2) | NED | 48.19 | | +5.310 |
| 9 | Lauren Teunissen (D2) | NED | 49.03 | | +6.150 |
| 10 | Linda Eggenkamp (M45) | NED | 49.16 | | +6.280 |
| 11 | Rikst van der Helm (Senior) | NED | 49.53 | SB | +6.650 |
| 12 | Manon van de Vliert-Scholte (M40) | NED | 49.90 | SB | +7.020 |
| 13 | Jamie Koudijs (B1) | NED | 51.00 | SB | +8.120 |
| 14 | Isa Molenaar (D2) | NED | 51.83 | | +8.950 |
| 15 | Pastel Riemersma (E2) | NED | 52.83 | SB | +9.950 |
| 16 | Marit Jansma (D1) | NED | 55.46 | SB | +12.580 |
| 17 | Ilse Baltussen (M40) | NED | 57.57 | | +14.690 |
| 18 | Gea Bakker (M45) | NED | 57.93 | PB | +15.050 |
| 19 | Marre Looijen (D2) | NED | 58.23 | SB | +15.350 |
| 20 | Marjolijn Theunissen (M50) | NED | 58.57 | PB | +15.690 |
| 21 | Rianne Servaas (B1) | NED | 59.01 | PB | +16.130 |
| 22 | Sophie Veen (B2) | NED | 59.02 | | +16.140 |
| 23 | Fleur van Nieuwenamerongen (B2) | NED | 59.56 | SB | +16.680 |
| 24 | Emma Kole (D2) | NED | 1:00.46 | | +17.580 |
| 25 | Mijke Theunissen (C1) | NED | 1:01.86 | SB | +18.980 |
| 26 | Floor Ger van der Pas (E2) | NED | 1:05.11 | SB | +22.230 |
| 27 | Linde Rijnders (D2) | NED | 1:06.00 | | +23.120 |
| DNS | Janneke Hennipman (A2) | NED | DNS | | - |