| 1 | Marieke Mast (Senior) | NED | 45.62 | SB | - |
| 2 | Femke Jager (Neo) | NED | 46.05 | | +0.430 |
| 3 | Dorian Schoonderwoerd (M40) | NED | 46.35 | | +0.730 |
| 4 | Fien de Jong (A1) | NED | 46.36 | PB | +0.740 |
| 5 | Lotte Mailoa (A1) | NED | 48.49 | SB | +2.870 |
| 6 | Wendy van de Ham (M40) | NED | 48.60 | | +2.980 |
| 7 | Marlous Prinzen (M30) | NED | 49.02 | SB | +3.400 |
| 8 | Simone Hoogendoorn (M30) | NED | 49.04 | SB | +3.420 |
| 9 | Wieke Leenhouts (C2) | NED | 49.08 | | +3.460 |
| 10 | Linda Krikke (M45) | NED | 49.10 | | +3.480 |
| 11 | Elise Groenendaal (Neo) | NED | 49.96 | | +4.340 |
| 12 | Janneke van de Kruijf-van Oort (M50) | NED | 51.10 | | +5.480 |
| 13 | Vera van Straten (B1) | NED | 51.14 | | +5.520 |
| 14 | Floor van Mourik (C1) | NED | 51.20 | | +5.580 |
| 15 | Milou Middelkoop (C2) | NED | 52.13 | | +6.510 |
| 16 | Britney Pieterse (B2) | NED | 52.41 | | +6.790 |
| 17 | Marleen.02 van Straten (C1) | NED | 54.27 | | +8.650 |
| 18 | Joelle Veerman (B1) | NED | 55.22 | PB | +9.600 |
| 19 | Madelief Middelweerd (C1) | NED | 56.21 | | +10.590 |
| 20 | Floortje Bouman (D2) | NED | 56.49 | SB | +10.870 |
| 21 | Anne de Vries (D2) | NED | 56.72 | | +11.100 |
| 22 | Eveline Zijderveld (C2) | NED | 58.52 | SB | +12.900 |
| 23 | Lotte Schalkwijk (D2) | NED | 1:01.94 | SB | +16.320 |
| 24 | Isa Meerveld (D1) | NED | 1:02.91 | SB | +17.290 |
| 25 | Kiki Scheel (C1) | NED | 1:05.11 | SB | +19.490 |
| 26 | Veerle van der Does (E2) | NED | 1:06.60 | | +20.980 |
| 27 | Medea Schalkwijk (E2) | NED | 1:07.80 | SB | +22.180 |
| 28 | Nienke de Bruin (F2) | NED | 1:10.33 | | +24.710 |